Recombinant TIM3 anticorps

N° du produit ABIN7072498

Cet anticorps Rat Monoclonal détecte spécifiquement TIM3 dans FACS, IHC, IF et BP. Il présente une réactivité envers Souris.

Aperçu rapide pour Recombinant TIM3 anticorps (ABIN7072498)

Antigène: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TIM3 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Blocking Peptide (BP)

Clone: 2C12

Détail du produit anti-TIM3 anticorps

Fonction: Anti-Tim-3 [2C12], Rat IgG1, kappa

Specificité: This antibody is specific for murine TIM-3, a Th1-specific cell surface protein. TIM-3 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. This antibody binds to the BALB/c allele of TIM-3 while reactivity to the C57Bl/6 allele is significantly weaker.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund's adjuvant.

Isotype: IgG1 kappa

Information d'application

Indications d'application: This antibody was used in FACS, immunohistochemistry, immunofluorescence and a murine experimental autoimmune encephalomyelitis model to study the effects of TIM-3 triggering in dendritic cells (Anderson et al., 2007, PMID: 18006747). Furthermore, this antibody has been used in multiple FACS analyses for diverse immuno-oncological applications, such as to delineate how TIM-3 signaling on cells of the innate immune system critically influences regulation of the adaptive immune response (Frisancho-Kiss et al., 2006), to demonstrate the reversal of T cell exhaustion and restoration of anti-tumor immunity upon co-targeting Tim-3 and PD-1 pathways (Sakuishi et al., 2010, PMID: 20819927), and to examine the therapeutic potential of dendritic cell based vaccines against malignant glioma (Dey et al., 2015). This antibody has also been used in immunohistochemistry to aid the elucidation of the effects of Th2 immune deviation on corneal allograft survival and possible mechanisms of graft rejection (Beauregard et al., 2005). In addition, employing this anti-TIM-3 antibody as part of the combination immuntherapy (anti-TIM3 + anti-PD-1 +/- anti-CTLA-4) has been shown to be effective against experimental and carcinogen-induced tumors in mice (Sakuishi et al., 2010, PMID: 20819927) (Ngiow et al., 2011, PMID: 21430066).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur TIM3

Antigène: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Autre désignation: Tim-3

Classe de substances: Virus

Sujet: CD366, HAVcr-2, HAVCR2, Hepatitis A virus cellular receptor 2 homolog, T-cell immunoglobulin and mucin domain containing 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIMD-3, TIM3, 8B.2C12

UniProt: Q8VIM0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints